Rna isolation kit invitrogen

Rna isolation kit invitrogen. The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. Results per page. Recover and enrich the transcriptome efficiently. The Ambion brand includes legacy Invitrogen products such as trusted TRIzol Reagents and PureLink purification The Invitrogen PureLink RNA Mini Kit provides rapid column-based purification of total RNA from a broad range of cell and tissue types. AM1912) or MagMAX Plant RNA Isolation Kit (Cat. Mix by inversion. Find the right RNA extraction kit or reagent to support your research. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA The Invitrogen™ PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. 5 x 106 to 108 tissue culture cells with one spin column in 20 minutes; Isolate more RNA than competitor mini spin columns: up to 1000 µg of total RNA from one column The remainder of the lysate is used for RNA isolation, using a procedure that combines the advantages of organic and solid-phase extraction, while avoiding the disadvantages of both. RNA extraction kits and reagents help labs to isolate, purify, and select for total RNA, transcriptome RNA, mRNA, microRNA and other small RNAs, and sequence-specific RNA. Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. Samples can be stored at 4°C for one month, at 25°C for one week, or at –20°C indefinitely. Proteinase K is provided separately for optional upfront Description. The PureLink RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction (see Figure 1). 2 mL of chloroform or 50 μL of 4-bromoanisole per 1 mL of TRIzolTM Reagent used for lysis, then securely cap the tube. The kit consists of two parts: Total Exosome Isolation Reagent (from plasma) and Proteinase K (20 mg/mL). Materials and methods Kits tested: RNA Extraction by Sample Type. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue. PureLink™ RNA Mini Kit. This means that users can process both small and large sample sizes with the same RNA isolation kit. The Monarch Total RNA Miniprep Kit can generate high quality RNA from as few as 100 HeLa cells. Whether you’re starting with cultured cells or or tissue samples, or working with plants, bacteria, or mammalian cells, Ambion RNA extraction reagents and kits offer the right product for your research. The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. Directions for purifying RNA from animal and plant cells using the PureLink™ RNA Mini Kit are described below. Features. RNAlater RNA Stabilization Solution is compatible with one-step RNA isolation methods, such as TRIzol Reagent; with glass binding methods, such as Qiagen's RNeasy or the Ambion RNAqueous kit; with acid phenol extraction methods, and with methods that use oligo(dT) selection of mRNA, such as the Ambion Poly(A)Purist kit. This kit is designed to provide consistent, efficient RNA recovery without sacrifi The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). Up to 1,000 ug of purified RNA from a single extraction. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The Ambion™ PureLink™ miRNA Isolation Kit is specifically designed to purify small RNA molecules, including miRNAs and siRNAs, from a variety of samples typically in less than 15 minutes (see Table 1). Depending on sample volume, the assay kit is designed to be accurate for initial RNA sample concentrations of 0. 1–1. Catalog number: 12183018A. No. The isolation protocol relies on base pairing between the polyA residues at the 3 The MagMAX™ mir Vana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The assay is performed at room temperature, and the signal is stable for approximately MagMAX Viral/Pathogen kits and reagents use magnetic bead-based technology to purify high-quality nucleic acid from a range of research sample types both manually and through automation. Learn about methods for genomic DNA extraction, isolation, and purification. Use 1 mL of 75% cold ethanol per 1 mL of TRIzol reagent used. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-25 μg from a single Tempus™ Blood RNA tube. The choice of RNA extraction kit or RNA isolation kit depends on your sample type, throughput required, or what equipment is available in the lab. The entire protocol can be done in spin tubes and spin columns. Higher yields of mRNA than comparable systems. Simplified, nontoxic RNA purification. The RNA precipitate forms a translucent gel-like pellet on the side and bottom of the tube. TRIzol reagents are offered in several ready-to-use formats. g. The Total Exosome RNA and Protein Isolation Kit is designed to isolate RNA and protein from enrichedexosome samples. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular Simple RNA Purification from Whole Blood. 2 to 200 ng/μL, providing a detection range of 4−200 ng. • Minimizes time required for RNA isolation by eliminating enzymatic and mechanical Nucleic acid isolation by organic extraction involves addition of phenol and guanidine isothiocyanate to separate the DNA, RNA, and proteins into different organic phases. 5–250 mg tissue. Organic extraction is a low-cost method, and with advanced reagents such as TRIzol, is a straightforward process requiring very little equipment. The PureLink DNase Set provides rapid and efficient removal of DNA from RNA which has been purified using PureLink RNA kits. Centrifuge the sample for 15 minutes at 12,000 × g at 4°C. 5 µg RNA was used in RT-PCR reactions with Ambion's RETROscript™ Kit. Find genomic DNA isolation kits. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue A. For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. Decant the supernatant to a clean, labeled 1. The kit utilizes a silica-based two-column system to enrich exclusively for small RNAs (see Figure 1). The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e. Add to cart. Protocol for Synthesizing Biotinylated aRNA. ( Lane 1) One Step RNA Isolation ( Lane 2 The MagMAX™Magnetic particle processor, one of the following:mirVana™Total RNA Isolation Kit is designed for isolation of total RNA, including microRNA, from a wide variety of sample matrices. RNA Structure - Function The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. For viral RNA isolation from biological fluids, such as serum, plasma, and swabs, we recommend using the MagMAXTM-96 Total RNA Isolation Kit (Cat #AM1835). Invitrogen technical resources—including technical notes, protocols, fundamentals, and more—demonstrate our wealth of expertise. The RNAqueous™-96 Kit is ideal for the Invitrogen™ Phasemaker™ Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of a TRIzol® Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA. Backed by 30 years of experience, our scientists and technical support teams are knowledge leaders in RNA research, and experts in RNA purification. 00 / 50 Preps. Obtain pure mRNA in typically 15 minutes. gov Works great on tough samples Less than a 1-hour protocol for even the toughest samples Add 0. If necessary, add1X PBS to a final volume of 200 µL before proceeding with the RNA isolation. The filter is then washed and the RNA is eluted in a small volume (50 μL or more) of elution solution. The mirVana miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 10 2 –10 7 cultured cells or 0. PureLink™ Total RNA Blood Kit is designed for purifying from 50 to 500 μl fresh whole blood or approximately 3. service@invitrogen. Purifying RNA for research & analysis. Incubate for 2–3 minutes. , from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. A33784) for RNA purification from plant tissues. Ribominus™ Transcriptome Isolation Kit (Yeast and Bacteria) RNAqueous™-96 RNA Isolation Kit Automation Protocol. The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible. Wash the RNA pellet once with 75% ice-cold ethanol. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. 5 mL microcentrifuge tube and set aside. Includes: Sufficient for up to 50 reactions and each reaction is capable of purifying total RNA for up to 10 slices of 10 μm-thick FFPE sections. The Invitrogen Ambion Poly(A)Purist MAG Kit employs the same hybridization and wash solutions as those in the Poly(A)Purist Kit but with the added ease, speed, and scalability of magnetic beads. 1g to 0. Archive tissues treated with RNA later solution at TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. The mixture separates into a lower red phenol-chloroform, and interphase, and a colorless upper aqueous phase. The kit can be used for clean-up of purified RNA from enzymatic reactions, desalting of RNA samples after phenol extraction or ethanol precipitation, and concentration of RNA. The kit allows recovery of both RNA and proteins from the same purified exosome preparation. The Ambion brand includes legacy Invitrogen products such as trusted TRIzol Reagents and PureLink purification Nucleic acid isolation by organic extraction involves addition of phenol and guanidine isothiocyanate to separate the DNA, RNA, and proteins into different organic phases. Invitrogen TRIzol products are referenced in thousands of journal publications, reflecting the trust that molecular biologists have placed in TRIzol Reagent to deliver high-quality, intact RNA from many kinds of biological materials. Our comprehensive products provide research scientists with the quality and performance they need. The Invitrogen RiboMinus Bacteria 2. 352. Procedural steps. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. , from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. High-quality RNA in less than 20 minutes. The Invitrogen PureLink nucleic acid purification kits are a family of purification products designed to meet the challenges posed by specific nucleic acid types, sample sources and volumes, throughput levels and downstream applications. 5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. Convenient syringe-based filtration; Compatibility with vacuum manifolds when processing multiple samples Mouse liver total RNA was isolated according to protocol by five different methods and 0. The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0. 5g of tissue or from 10 7 to 10 8 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. PCR reactions (minus-RT) were performed with 5 µg RNA. Archive tissues treated with RNA later solution at The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0. These kits are based on either silica-membrane, anion exchange resin, or Small organs such as rat kidney, liver, and spleen can be stored whole in RNA later solution. RNA Purification Protocols. The Ambion brand includes legacy Invitrogen products such as trusted TRIzol Reagents and PureLink purification RiboMinus rRNA depletion kit for bacteria. The polymer in Phasemaker Tubes is heavier than the aqueous phase of the TRIzol Reagent RNAlater RNA Stabilization Solution is compatible with one-step RNA isolation methods, such as TRIzol Reagent; with glass binding methods, such as Qiagen's RNeasy or the Ambion RNAqueous kit; with acid phenol extraction methods, and with methods that use oligo(dT) selection of mRNA, such as the Ambion Poly(A)Purist kit. RNA Extraction by Sample Type. Total RNA extraction kits are optimized to purify a broader range of RNA, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and micro RNA (miRNA or microRNA)—not just mRNA. 10 µl of each reaction was electrophoresed on a 2% agarose gel and stained with EtBr. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples: Invitrogen Corporation 1600 Faraday Avenue Carlsbad, CA 92008 T: 1 760 603 7200 F: 1 760 602 6500 E: tech. Total RNA can be isolated using any of the available isolation kits such as the RNeasy ® Mini Kit (Qiagen, Hilden, Germany) following the instructions suggested by the manufacturer. Prepare mRNA that is suitable for nearly every downstream application. Our full suite of products is backed by scientists and engineers and is The Quant-iT microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. 5 x 10 6 leukocytes (white blood cells) in less than 1 hour. Dynabeads™ magnetic beads are uniform in size and highly mobile in solution (see figure). The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e. The isolation protocol relies on base pairing between the polyA residues at the 3 The Invitrogen ™ TRIzol Reagent is the most cited organic reagent for isolation of RNA, DNA, and protein 100,000 150,000 50,000 200,000 1990 1995 2000 2005 2010 2015 2019 Citations 161,173 1990–2019 Year Citations According to https://ncbi. This enables the bead capture surface to quickly and continuously interact with the entire Oct 9, 2020 · To achieve this objective, five RNA extraction methods, i. RNA extraction and isolation methods comparison chart. For detailed instructions, and protocols for isolating RNA from tissue, blood, bacteria, yeast, liquid samples, and on-column DNAse treatment, download the manual from www. Clean up and concentrate RNA amounts ranging from pg to 100 μg in volumes as small as 10 μL. It can be used to isolate total RNA from a variety of gram-negative and gram-positive bacteria. The PureLink RNA Mini Kit is recommended for use with the Homogenizer designed to homogenize cell The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The PureLink™ Genomic Plant DNA Purification Kit allows efficient isolation of total DNA in about 40 minutes from a variety of plant samples, such as spinach, tomato, soy, wheat, Arabidopsis, and mushroom. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions. Isolated mRNA has minimal ribosomal RNA and genomic DNA contamination. The mirVana™ miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 10 2 –10 7 cultured cells or 0. The PureLink™ Genomic Plant DNA Purification Kit is suitable for isolating DNA from chloroplasts and a variety of plant tissues (Figure . DNA- free ™ DNA removal reagents RNA Extraction by Sample Type. • Typical yields are >50% of those of unfixed tissue. 0 Transcriptome Isolation Kit is designed to capture rRNA from a wide variety of prokaryotes. The Total Exosome Isolation Reagent enables simple and reliable concentration of intact exosomes from plasma samples with a protocol that is scalable depending on your sample size. Isolate small RNA-containing total RNA and native protein from the same sample; Start with 100–10 7 cultured cells or up to 100 mg of many mammalian tissues The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 µg). Background. nih. The MagMAX™-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. gov Works great on tough samples Less than a 1-hour protocol for even the toughest samples The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. Accessory products The Plant RNA Isolation Aid is designed for use with the RNAqueous Kit (Cat. The transcription reaction is mixed with Binding Solution, passed over an The PureLink FFPE RNA Isolation Kit provides a simple, reliable, and rapid method to isolate high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens. TRIZOL kit (Invitrogen), Plant RNeasy mini kit (Qiagen), Furtado 6 method, CTAB-LiCl method 3, and our modified SDS-LiCl method, were Documents. Introduction. Leukocyte Enrichment for Improved RNA Purification. The FastTrack® MAG mRNA Isolation Kit offers the following advantages: Isolates high-quality mRNA in less than 1 hour from total RNA and 1. The reagents and consumables included in the TempusTM Spin RNA Isolation Kit allow you to isolate 2 to 8 μg of high quality RNA per milliliter of whole blood. The MagMAXTM-96 Total RNA Isolation Kit can also be used for isolation of viral RNA from these sample types. e. Resuspend pelleted cells in a small amount of PBS before adding 5–10 volumes of RNA later solution. 15. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with just trace amounts of residual genomic DNA contamination. com PureLink™ FFPE Total RNA Isolation Kit For rapid purification of total RNA from formalin-fixed, paraffin-embedded (FFPE) tissues Isolation of RNA takes about an hour. com or by contacting Technical Support. The PureLink FFPE RNA Isolation Kit provides a simple and rapid method for isolating total RNA from formalin-fixed, paraffin-embedded (FFPE) samples with high-quality results. The isolated mRNA is suitable for use in all downstream applications. Enrich for small RNA The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction. Accurate —optimized hybridization chemistry designed for whole transcriptome RNA-seq. Protocol for Trichloroacetic Acid (TCA) Precipitation. Description. • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon. The kit uses MagMAX™magnetic-bead technology, ensuring reproducible recovery of high-quality RNA that is suitable for a broad range of applications PureLink Purification Solutions. 5 hours from cells or tissue. The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink. Final Product: mRNA. The Dynabeads™ mRNA Purification Kit contains magnetic beads for the isolation of the mRNA transcriptome from any total eukaryotic RNA preparation (see figure). The Dynabeads mRNA Purification Kit is designed to specifically target, capture, and purify mRNA molecules from total RNA preparations. • Specifically designed to purify viral RNA and DNA from ≤500 μL cell-free samples within 45 min. RNAs greater than approximately 200 The Invitrogen PureLink RNA Mini Kit provides rapid column-based purification of total RNA from a broad range of cell and tissue types. Protocol for 3' End Labeling RNA. Invitrogen's Plant RNA Isolation Aid contains polyvinylpyrrolidone (PVP), a high molecular weight polymer that binds to contaminants such as polyphenolics and polysaccharides that are This means that users can process both small and large sample sizes with the same RNA isolation kit. RiboPureTM-Bacteria is a rapid RNA isolation kit which combines dis-ruption of bacterial cell walls with Zirconia Beads, phenol extraction of the lysate, and glass-fiber filter purification of the RNA. The Arcturus PicoPure RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. The Invitrogen PureLink RNA Mini Kit provides rapid column-based purification of total RNA from a broad range of cell and tissue types. To learn more about these methods, see Nucleic Acid Extraction. The RNAqueous™-96 Kit is ideal for the The PureLink Viral RNA/DNA Mini Kit provides the following advantages: • Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination. Documents. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also Prior to RNA isolation, cells have to be washed with Ca 2+ - and Mg 2+-free PBS (Gibco, Invitrogen) and lysed directly in the culture dish. In-house research, as The Invitrogen PureLink RNA Mini Kit provides rapid column-based purification of total RNA from a broad range of cell and tissue types. Ambion is now Invitrogen. Our kits are recommended for viral nucleic acid The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. Simply dilute the reagent 1:200, load 200 μL into the wells of a microplate, add 1–20 μL of sample, mix, and read the fluorescence. Exosomal RNA recovered using the Total Exosome RNA and Protein Isolation Kit can be analyzed by qRT-PCR using Applied Biosystems TaqMan Assays, RNA sequencing, or next The Qubit RNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The protocol typically takes only 45 minutes, and the kit performs from 8 large enrichment reactions (up to 1 mg total RNA input) to 80 small The Invitrogen ™ TRIzol Reagent is the most cited organic reagent for isolation of RNA, DNA, and protein 100,000 150,000 50,000 200,000 1990 1995 2000 2005 2010 2015 2019 Citations 161,173 1990–2019 Year Citations According to https://ncbi. nlm. Simplified, nontoxic RNA purification Description. RNA Wash. Recommended for use with the RNAqueous Kit for purification of total RNA from plant tissues. The RNAqueous™-96 Kit is ideal for the Catalog number: 12183018A. • Uses two technologies for maximum RNA yield and purity. In-house research, as PureLink™ FFPE RNA単離キットは、市販の他のRNA精製システムと比べて高い収量と純度を提供し、1 kbを超えるRT-PCR生成物に対応します（図2）。 さらに、PureLink™ FFPE RNA単離キットは、精製RNAサンプルのゲノムDNA汚染を最小限に抑え、DNase消化のオプションを提供 available kits—the Invitrogen™ PureLink RNA Mini Kit and Qiagen ™ RNeasy Mini Kit—in the extraction of RNA from heart, liver, and brain tissue of mice. Protocol for 5' End Labeling RNA. invitrogen. Compatible cell and tissue types include mammalian cells, plant cells, yeast cells, bacteria, and blood. com For country-specific contact information visit our web site at www. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. For RNA isolation, up to 200 L of µ resuspended exosome material can be used. Key Features of the TRIzol™ Max™ Bacterial RNA Isolation Kit: • Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria. To help save time and increase reproducibility, combine with KingFisher instruments for automated purification. As shown in Table 1, a larger percentage of organ tissue can be purified with a single PureLink column than with a Qiagen column. Total RNA was isolated using the Monarch Total RNA Miniprep Kit (NEB #T2010) from varying amounts of HeLa cells over 5 orders of magnitude and eluted in 100 µl of nuclease-free water. The individual products within the TRIzol reagent RNA analysis—Exosomal RNA and membrane components can be labeled using fluorescent dyes, while unincorporated dye can be easily depleted with Invitrogen spin columns. Small organs such as rat kidney, liver, and spleen can be stored whole in RNA later solution. The kit utilizes the strong lysis capability of TRIzol® Reagent, followed by a convenient and time–saving The TempusTM Blood RNA Tube, enables you to draw blood directly into a reagent that stabilizes RNA at room temperature for up to five days. One bottle of 10 mL Invitrogen reagent is provided. Advantages of the MEGAclear™ Kit: The protocol is quick and easy. Purify RNA from up to 10 mg of tissue or 10 5 cells. RNA purified using the kit is ideal for PCR and RT-PCR applications. Flexible —pan-prokaryote probes for bacterial rRNA depletion are compatible with The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000μg of purified RNA from a single extraction. The TRIzol® Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high–quality total RNA from a wide variety of samples, including animal and plant cells and tissue, bacteria, and yeast. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Process sample inputs from 5–200 mg of animal/plant tissue or from 0. It is suitable for studies of RNA expression (specifically miRNA), processing, or function. Samples were analyzed on a Bioanalyzer Pico chip, with RIN values The RNAqueous Kit is for phenol-free total RNA isolation from 0. vn xo lp go vu qz zw xx xx le